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Literature summary for 4.2.1.83 extracted from
- Purification and properties of gamma-oxalomesaconate hydratase from Pseudomonas ochraceae grown with phthalate (1985), Biochem. Biophys. Res. Commun., 128, 271-277.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Pseudomonas straminea |  |
| cysteine | - |
Pseudomonas straminea | |
| DTT | - |
Pseudomonas straminea | |
| reduced glutathione | - |
Pseudomonas straminea | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | - |
Pseudomonas straminea | |
| CuSO4 | - |
Pseudomonas straminea | |
| HgCl2 | - |
Pseudomonas straminea | |
| iodoacetamide | weak | Pseudomonas straminea | |
| NEM | - |
Pseudomonas straminea | |
| NiCl2 | - |
Pseudomonas straminea | |
| PCMB | - |
Pseudomonas straminea | |
| ZnCl2 | - |
Pseudomonas straminea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0064 | - |
(E)-3-Carboxy-5-oxo-2-hex-2-enedioic acid | pH 8.0, 24°C | Pseudomonas straminea | |
| 0.014 | - |
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate | pH 8.0, 24°C | Pseudomonas straminea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no Fe2+- or Mn2+-dependent activation in the presence of DTT under anaerobic conditions | Pseudomonas straminea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, SDS-PAGE | Pseudomonas straminea |
| 68000 | - |
gel filtration | Pseudomonas straminea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas straminea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas straminea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 108 | - |
- |
Pseudomonas straminea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 1 month | Pseudomonas straminea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O | i.e. (E)-4-oxobut-1-ene-1,2,4-tricarboxylate, 4-oxalmesaconate, highly specific | Pseudomonas straminea | 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate | i.e. 4-carboxy-4-hydroxy-2-oxoadipic acid, gamma-oxalcitramalate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 37000, SDS-PAGE | Pseudomonas straminea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Pseudomonas straminea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Pseudomonas straminea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9.6 | 50% of activity maximum at pH 7.2 and 9.6 | Pseudomonas straminea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas straminea | isoelectric focusing | - |
4.9 |
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