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Literature summary for 4.2.1.82 extracted from

  • Johnsen, U.; Dambeck, M.; Zaiss, H.; Fuhrer, T.; Soppa, J.; Sauer, U.; Schoenheit, P.
    D-xylose degradation pathway in the halophilic archaeon Haloferax volcanii (2009), J. Biol. Chem., 284, 27290-27303.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
xylose xylonate dehydratase activity is induced 2fold in xylose-grown cells Haloferax volcanii

Cloned(Commentary)

Cloned (Comment) Organism
PCR product cloned into pET19b. The resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain. In-frame deletion mutant amplified and ligated into pTA131, sequenced, and transformed in Haloferax volcanii H26 DELTApyrE2 Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
D-gluconate at 42°C, in 0.1 M Tris-HCl buffer, pH 7.5, 2.5 M KCl, 50 mM MgCl2 Haloferax volcanii
0.55
-
D-xylonate at 42°C, in 0.1 M Tris-HCl buffer, pH 7.5, 2.5 M KCl, 50 mM MgCl2 Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
8 * 45500, sequence analysis. 8 * 52000, gel filtration Haloferax volcanii
52000
-
8 * 45500, sequence analysis. 8 * 52000, gel filtration Haloferax volcanii
340000
-
gel filtration Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GP40
-
-

Purification (Commentary)

Purification (Comment) Organism
by centrifugation, gel filtration, ammonium sulfate precipitation and ultrafiltration, 100fold. Recombinant XAD purified by Ni-NTA chromatography Haloferax volcanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
100fold purified enzyme, with D-xylonate or D-gluconate as substrate Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate XAD catalyzes the dehydration of D-gluconate at a similar catalytic efficiency as compared with D-xylonate Haloferax volcanii 2-dehydro-3-deoxy-D-gluconate + H2O
-
?
D-xylonate XAD catalyzes the dehydration of D-xylonate at a similar catalytic efficiency as compared with D-gluconate Haloferax volcanii 2-dehydro-3-deoxy-D-arabinonate + H2O
-
?
additional information D-galactonate is not utilized Haloferax volcanii ?
-
?

Subunits

Subunits Comment Organism
homooctamer 8 * 45500, sequence analysis. 8 * 52000, gel filtration Haloferax volcanii

Synonyms

Synonyms Comment Organism
D-xylonate dehydratase
-
Haloferax volcanii
XAD
-
Haloferax volcanii
xylonate dehydratase
-
Haloferax volcanii

General Information

General Information Comment Organism
malfunction in-frame deletion mutant has lost its ability to grow on xylose, but it shows the same phenotype as the wild-type during growth on glucose Haloferax volcanii