Activating Compound | Comment | Organism | Structure |
---|---|---|---|
xylose | xylonate dehydratase activity is induced 2fold in xylose-grown cells | Haloferax volcanii |
Cloned (Comment) | Organism |
---|---|
PCR product cloned into pET19b. The resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain. In-frame deletion mutant amplified and ligated into pTA131, sequenced, and transformed in Haloferax volcanii H26 DELTApyrE2 | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
D-gluconate | at 42°C, in 0.1 M Tris-HCl buffer, pH 7.5, 2.5 M KCl, 50 mM MgCl2 | Haloferax volcanii | |
0.55 | - |
D-xylonate | at 42°C, in 0.1 M Tris-HCl buffer, pH 7.5, 2.5 M KCl, 50 mM MgCl2 | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45500 | - |
8 * 45500, sequence analysis. 8 * 52000, gel filtration | Haloferax volcanii |
52000 | - |
8 * 45500, sequence analysis. 8 * 52000, gel filtration | Haloferax volcanii |
340000 | - |
gel filtration | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | D4GP40 | - |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation, gel filtration, ammonium sulfate precipitation and ultrafiltration, 100fold. Recombinant XAD purified by Ni-NTA chromatography | Haloferax volcanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
100fold purified enzyme, with D-xylonate or D-gluconate as substrate | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate | XAD catalyzes the dehydration of D-gluconate at a similar catalytic efficiency as compared with D-xylonate | Haloferax volcanii | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
D-xylonate | XAD catalyzes the dehydration of D-xylonate at a similar catalytic efficiency as compared with D-gluconate | Haloferax volcanii | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
additional information | D-galactonate is not utilized | Haloferax volcanii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 45500, sequence analysis. 8 * 52000, gel filtration | Haloferax volcanii |
Synonyms | Comment | Organism |
---|---|---|
D-xylonate dehydratase | - |
Haloferax volcanii |
XAD | - |
Haloferax volcanii |
xylonate dehydratase | - |
Haloferax volcanii |
General Information | Comment | Organism |
---|---|---|
malfunction | in-frame deletion mutant has lost its ability to grow on xylose, but it shows the same phenotype as the wild-type during growth on glucose | Haloferax volcanii |