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Literature summary for 4.2.1.82 extracted from

  • Dahms, A.S.; Donald, A.
    D-xylo-aldonate dehydratase (1982), Methods Enzymol., 90, 302-305.
    View publication on PubMed
Search for more literature for 4.2.1.82

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is induced by growth on D-xylose, but not by D-gluconate, D-glucose, L-arabinose or D-fucose Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Pseudomonas sp.
additional information not inhibited by thiols, iodoacetic acid, p-mercuribenzoate or N-ethylmaleimide, all at 2 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.77
-
 D-gluconate
-
 Pseudomonas sp.
2.98
-
 D-xylonate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
FeSO4 addition of 5 mM to the EDTA-inhibited enzyme results in 75% of the original activity Pseudomonas sp.
MgCl2 addition of 5 mM to the EDTA-inhibited enzyme results in 100% of the original activity Pseudomonas sp.
MgSO4 addition of 5 mM to the EDTA-inhibited enzyme results in 100% of the original activity Pseudomonas sp.
Mn2+ addition of 5 mM to the EDTA-inhibited enzyme results in 13% of the original activity Pseudomonas sp.
additional information absolute requirement for a divalent cation, no reactivation of EDTA-inhibited enzyme by CuSO4, CoCl2, ZnSO4 and NiCl2, all at 5 mM Pseudomonas sp.
PbCl2 addition of 5 mM to the EDTA-inhibited enzyme results in 50% of the original activity Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism ?
-
 ?
additional information Pseudomonas sp. MSU-1 enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. MSU-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 21fold Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.38
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
frozen state, several months, stable Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate at 55% of the rate with D-xylonate Pseudomonas sp. 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
D-gluconate at 55% of the rate with D-xylonate Pseudomonas sp. MSU-1 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
D-xylonate
-
 Pseudomonas sp. 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
D-xylonate
-
 Pseudomonas sp. MSU-1 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
additional information not: D- and L-arabonate, D- and L-fuconate, 6-iodo-6-deoxygalactonate, D- and L-mannonate, D- and L-galactonate, L-gluconate, D-ribonate, D-glucuronate, D-galacturonate, D-galactarate Pseudomonas sp. ?
-
 ?
additional information enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism Pseudomonas sp. ?
-
 ?
additional information not: D- and L-arabonate, D- and L-fuconate, 6-iodo-6-deoxygalactonate, D- and L-mannonate, D- and L-galactonate, L-gluconate, D-ribonate, D-glucuronate, D-galacturonate, D-galactarate Pseudomonas sp. MSU-1 ?
-
 ?
additional information enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism Pseudomonas sp. MSU-1 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 assay at Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life: 14 min Pseudomonas sp.
70
-
 half-life: 2 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in 50 mM Bicine Pseudomonas sp.