Literature summary for 4.2.1.80 extracted from

Pollard, J.R.; Bugg, T.D.H.
Purification, characterization and reaction mechanism of monofunctional 2-hydroxypentadienoic acid hydratase from Escherichia coli (1998), Eur. J. Biochem., 251, 98-106.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Escherichia coli
sodium oxalate	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.041	-	2-hydroxypentadienoic acid	pH 6	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	reqirement of divalent cations	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	gel filtration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
538	-	MonoQ pool	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxypentadienoic acid	-	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
2-hydroxypentadienoic acid hydratase	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0049	-	sodium oxalate	pH 6	Escherichia coli

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Release 2023.1 (January 2023)