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Literature summary for 4.2.1.79 extracted from

  • Grimek, T.L.; Escalante-Semerena, J.C.
    The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo (2004), J. Bacteriol., 186, 454-462.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.79

Activating Compound

Activating Compound Comment Organism Structure
propionate induces enzyme expression, enzyme is required for growth on propionate Vibrio cholerae serotype O1
propionate induces enzyme expression, enzyme is required for growth on propionate Shewanella oneidensis
PrpF seems to be an accessory protein required to prevent oxidative damage of the Fe/S center of active AcnD enzyme, or is involved in synthesis or repair of the Fe/S cluster present in AcnD Vibrio cholerae serotype O1
PrpF seems to be an accessory protein required to prevent oxidative damage of the Fe/S center of active AcnD enzyme, or is involved in synthesis or repair of the Fe/S cluster present in AcnD Shewanella oneidensis

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged AcnD and PrpF in Escherichia coli BL21(DE3), functional complementation of an enzyme-deficient mutant of Salmonella enterica only by expression of both AcnD and PrpF Shewanella oneidensis
structural genetic organization of prp operon Vibrio cholerae serotype O1

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ AcnD enzyme possesses 1 unstable iron-sulfur center per monomer, absolutely required for activity, can be reconstituted by Fe2+ under reducing conditions Vibrio cholerae serotype O1
Fe2+ AcnD enzyme possesses 1 unstable iron-sulfur center per monomer, absolutely required for activity, can be reconstituted by Fe2+ under reducing conditions Shewanella oneidensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate Vibrio cholerae serotype O1
-
 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
2-Hydroxybutane-1,2,3-tricarboxylate Shewanella oneidensis
-
 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Shewanella oneidensis
-
 gene acnD and prpF are both needed for full enzyme activity, they exist in place of or in addition to prpD
-
Vibrio cholerae serotype O1
-
 gene acnD and prpF are both needed for full enzyme activity, they exist in place of or in addition to prpD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged AcnD and PrpF from Escherichia coli BL21(DE3) Shewanella oneidensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Shewanella oneidensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate
-
 Vibrio cholerae serotype O1 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
2-Hydroxybutane-1,2,3-tricarboxylate
-
 Shewanella oneidensis (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate, best substrate Vibrio cholerae serotype O1 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate ?
2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate, best substrate Shewanella oneidensis (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate ?
citrate
-
 Vibrio cholerae serotype O1 cis-aconitate + H2O
-
 ?
citrate
-
 Shewanella oneidensis cis-aconitate + H2O
-
 ?
additional information 2-methylisocitrate and isocitrate are no substrates for AcnD Vibrio cholerae serotype O1 ?
-
 ?
additional information 2-methylisocitrate and isocitrate are no substrates for AcnD Shewanella oneidensis ?
-
 ?

Synonyms

Synonyms Comment Organism
PrpD
-
 Vibrio cholerae serotype O1
PrpD
-
 Shewanella oneidensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Shewanella oneidensis