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Literature summary for 4.2.1.79 extracted from

  • Tabuchi, T.; Aoki, H.; Uchiyama, H.; Nakahara, T.
    2-Methylcitrate dehydratase, a new enzyme functioning at the methylcitric acid cycle of propionate metabolism (1981), Agric. Biol. Chem., 45, 2823-2929.
No PubMed abstract available
Search for more literature for 4.2.1.79

General Stability

General Stability Organism
unstable in Tris-HCl buffer Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate Yarrowia lipolytica methylcitric acid cycle, propionyl-CoA oxidation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
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 formerly Saccaromycopsis lipolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O r Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate r ?
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O r Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate ?
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate r ?
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate ?
2-Hydroxybutane-1,2,3-tricarboxylate r Yarrowia lipolytica (Z)-But-2-ene-1,2,3-tricarboxylate + H2O r ?
2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate Yarrowia lipolytica (Z)-But-2-ene-1,2,3-tricarboxylate + H2O r ?
2-Hydroxybutane-1,2,3-tricarboxylate methylcitric acid cycle, propionyl-CoA oxidation Yarrowia lipolytica ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 40
-
 Yarrowia lipolytica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45
-
 Yarrowia lipolytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, stable Yarrowia lipolytica
60
-
 30 min, complete inactivation Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Yarrowia lipolytica

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5
-
 Yarrowia lipolytica

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5
-
 Yarrowia lipolytica