Literature summary for 4.2.1.78 extracted from

Lichman, B.R.; Zhao, J.; Hailes, H.C.; Ward, J.M.
Enzyme catalysed Pictet-Spengler formation of chiral 1,1-disubstituted- and spiro-tetrahydroisoquinolines (2017), Nat. Commun., 8, 14883 .

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Application

Application	Comment	Organism
synthesis	the enzyme can catalyse the Pictet-Spengler reaction between dopamine and unactivated ketones, thus facilitating the facile biocatalytic generation of 1,1'-disubstituted tetrahydroisoquinolines. Variants of the enzyme showing improved conversions are identified and used to synthesize novel chiral 1,10-disubstituted and spiro-tetrahydroisoquinolines	Thalictrum flavum

Protein Variants

Protein Variants	Comment	Organism
A79F	improved turnover of ketones, increase in side-chain steric interactions	Thalictrum flavum
A79I	improved turnover of ketones, increase in side-chain steric interactions	Thalictrum flavum

Organism

Organism	UniProt	Comment	Textmining
Thalictrum flavum	Q67A25	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-hydroxyphenylacetone + dopamine	-	Thalictrum flavum	1-(4-hydroxybenzyl)-1-methyl-1,2,3,4-tetrahydroisoquinoline-6,7-diol	-	?

Synonyms

Synonyms	Comment	Organism
TfNCS	-	Thalictrum flavum

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Release 2023.1 (January 2023)