Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fungal elicitor of Bortrytis sp. | isolated elicitor induces the enzyme activity in cell suspension culture 20fold | Papaver somniferum | |
additional information | enzyme is not affected by Mg2+ and Ca2+ | Papaver somniferum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by several benzylisochinoline alkaloids, enzyme is not affected by Mg2+ and Ca2+ | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, sigmoidal saturation kinetics for dopamine, Hill coeffcient 1.84, cooperativity between the dopamine binding sites on each subunit | Papaver somniferum | |
additional information | - |
additional information | kinetics, sigmoidal saturation kinetics for dopamine, Hill coeffcient 2.16, cooperativity between the dopamine binding sites on each subunit | Thalictrum flavum ssp. glaucum | |
additional information | - |
additional information | kinetics, sigmoidal saturation kinetics for dopamine, Hill coeffcient 2.19, cooperativity between the dopamine binding sites on each subunit | Eschscholzia californica | |
0.42 | - |
4-hydroxyphenylacetaldehyde | pH 7.0, 37°C, hyperbolic saturation kinetics | Thalictrum flavum ssp. glaucum | |
0.66 | - |
4-hydroxyphenylacetaldehyde | pH 7.0, 37°C, hyperbolic saturation kinetics | Eschscholzia californica | |
1 | - |
4-hydroxyphenylacetaldehyde | pH 7.0, 37°C, hyperbolic saturation kinetics | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | Eschscholzia californica | first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux | (S)-Norcoclaurine + H2O | - |
ir | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | Papaver somniferum | first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux | (S)-Norcoclaurine + H2O | - |
ir | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | Thalictrum flavum ssp. glaucum | first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux | (S)-Norcoclaurine + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eschscholzia californica | - |
- |
- |
no activity in Catharanthus roseus | - |
- |
- |
no activity in Nicotiana tabacum | - |
- |
- |
Papaver somniferum | - |
cv. Marianne | - |
Thalictrum flavum ssp. glaucum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetaldehyde + dopamine = (S)-norcoclaurine + H2O | the reaction makes a six-membered ring by forming a bond between C-6 of the 3,4-dihydroxyphenyl group of the dopamine and C-1 of the aldehyde in the imine formed between the substrates, the product is the precursor of the benzylisoquinoline alkaloids in plants, the enzyme, formerly known as (S)-norleudanosoline synthase, will also catalyse the reaction of 4-(2-aminoethyl)benzene-1,2-diol + (di-3,4-hydroxyphenyl)acetaldehyde to form (S)-norlaudanosoline, but this alkaloid has not been found to occur in plants, cooperativity between substrate binding sites | Eschscholzia californica | |
4-hydroxyphenylacetaldehyde + dopamine = (S)-norcoclaurine + H2O | the reaction makes a six-membered ring by forming a bond between C-6 of the 3,4-dihydroxyphenyl group of the dopamine and C-1 of the aldehyde in the imine formed between the substrates, the product is the precursor of the benzylisoquinoline alkaloids in plants, the enzyme, formerly known as (S)-norleudanosoline synthase, will also catalyse the reaction of 4-(2-aminoethyl)benzene-1,2-diol + (di-3,4-hydroxyphenyl)acetaldehyde to form (S)-norlaudanosoline, but this alkaloid has not been found to occur in plants, cooperativity between substrate binding sites | Papaver somniferum | |
4-hydroxyphenylacetaldehyde + dopamine = (S)-norcoclaurine + H2O | the reaction makes a six-membered ring by forming a bond between C-6 of the 3,4-dihydroxyphenyl group of the dopamine and C-1 of the aldehyde in the imine formed between the substrates, the product is the precursor of the benzylisoquinoline alkaloids in plants, the enzyme, formerly known as (S)-norleudanosoline synthase, will also catalyse the reaction of 4-(2-aminoethyl)benzene-1,2-diol + (di-3,4-hydroxyphenyl)acetaldehyde to form (S)-norlaudanosoline, but this alkaloid has not been found to occur in plants, cooperativity between substrate binding sites | Thalictrum flavum ssp. glaucum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Eschscholzia californica | - |
cell suspension culture | - |
Papaver somniferum | - |
cell suspension culture | - |
Thalictrum flavum ssp. glaucum | - |
plant | all organs especially in root and stem | Papaver somniferum | - |
root | highest activity level | Papaver somniferum | - |
seed | germinating | Papaver somniferum | - |
seedling | - |
Papaver somniferum | - |
stem | - |
Papaver somniferum | - |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme, 7 days, no loss of activity | Papaver somniferum |
4°C, purified enzyme, 7 days, 75% loss of activity | Papaver somniferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | 4-(2-aminoethyl)benzene-1,2-diol is dopmaine | Eschscholzia californica | (S)-Norcoclaurine + H2O | - |
ir | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | 4-(2-aminoethyl)benzene-1,2-diol is dopmaine | Papaver somniferum | (S)-Norcoclaurine + H2O | - |
ir | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | 4-(2-aminoethyl)benzene-1,2-diol is dopmaine | Thalictrum flavum ssp. glaucum | (S)-Norcoclaurine + H2O | - |
ir | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux | Eschscholzia californica | (S)-Norcoclaurine + H2O | - |
ir | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux | Papaver somniferum | (S)-Norcoclaurine + H2O | - |
ir | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux | Thalictrum flavum ssp. glaucum | (S)-Norcoclaurine + H2O | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
NCS | - |
Eschscholzia californica |
NCS | - |
Papaver somniferum |
NCS | - |
Thalictrum flavum ssp. glaucum |
norcoclaurine synthase | - |
Eschscholzia californica |
norcoclaurine synthase | - |
Papaver somniferum |
norcoclaurine synthase | - |
Thalictrum flavum ssp. glaucum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | - |
Eschscholzia californica |
45 | 55 | - |
Thalictrum flavum ssp. glaucum |
50 | - |
- |
Papaver somniferum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 57 | half-maximal activities at 37°C and 57°C | Papaver somniferum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
instable above 55°C | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Eschscholzia californica |
6.5 | 7 | - |
Papaver somniferum |
6.5 | 7 | - |
Thalictrum flavum ssp. glaucum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9 | 50% of maximal activity at pH 6.2, 68% of maximal activity at pH 9.0 | Papaver somniferum |