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Literature summary for 4.2.1.75 extracted from
- Mutagenesis identifies a conserved tyrosine residue important for the activity of uroporphyrinogen III synthase from Anacystis nidulans (2002), FEBS Lett., 525, 25-28.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression as N-terminally his-tagged enzyme in Escherichia coli | Synechococcus elongatus PCC 7942 = FACHB-805 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D112G | site-directed mutagenesis, 50% reduced activity compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
| R15Q | site-directed mutagenesis, unaltered activity level compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
| S68A | site-directed mutagenesis, unaltered activity level compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
| T100A | site-directed mutagenesis, 75% reduced activity compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
| Y166F | site-directed mutagenesis, more than 99% reduced activity compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli | Synechococcus elongatus PCC 7942 = FACHB-805 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| hydroxymethylbilane = uroporphyrinogen III + H2O | in the presence of hydroxymethylbilane synthase EC 4.3.1.8, enzyme forms uroporphyrinogen III from porphobilinogen, Tyr166 is essential for catalysis and/or substrate binding | Synechococcus elongatus PCC 7942 = FACHB-805 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.25 | - |
purified recombinant mutant Y166F | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 40 | - |
purified recombinant wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxymethylbilane | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | uroporphyrinogen-III + H2O | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| U3S | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| Uroporphyrinogen III synthase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Synechococcus elongatus PCC 7942 = FACHB-805 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Synechococcus elongatus PCC 7942 = FACHB-805 |
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Release 2023.1 (January 2023)
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