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Literature summary for 4.2.1.68 extracted from
- L.Fuconate dehydratase: purification and properties of the enzyme from Pullularia pullulans (1991), Arq. Biol. Tecnol., 34, 537-553.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| very unstable, not stabilized by MgCl2 or EDTA | Aureobasidium pullulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-arabonate | 10 mM, 100% inhibition | Aureobasidium pullulans |  |
| D-arabono-1,5-lactone | - |
Aureobasidium pullulans | |
| D-galactonate | 10 mM, 100% inhibition | Aureobasidium pullulans | |
| D-gluconate | 10 mM, 100% inhibition | Aureobasidium pullulans | |
| EDTA | 10 mM, 100% inhibition | Aureobasidium pullulans | |
| HgCl2 | 0.25 mM, ca. 90% inhibition | Aureobasidium pullulans | |
| iodoacetate | 2.5 mM, 90% inhibition | Aureobasidium pullulans | |
| L-Rhamnonate | 10 mM, 95% inhibition | Aureobasidium pullulans | |
| N-ethylmaleimide | 1 mM, 81% inhibition | Aureobasidium pullulans | |
| p-chloromercuribenzoate | 0.25 mM, 79% inhibition | Aureobasidium pullulans | |
| ZnSO4 | 5 mM, 78% inhibition | Aureobasidium pullulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
L-fuconate | - |
Aureobasidium pullulans | |
| 3.3 | - |
L-galactonate | - |
Aureobasidium pullulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
gel filtration | Aureobasidium pullulans |
| 104000 | - |
sucrose density ultracentifugation | Aureobasidium pullulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Fuconate | Aureobasidium pullulans | catabolism of L-fucose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aureobasidium pullulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aureobasidium pullulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.1 | - |
- |
Aureobasidium pullulans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 30% loss of activity in 1 month | Aureobasidium pullulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-fuconate | - |
Aureobasidium pullulans | 2-dehydro-3-deoxy-L-fuconate + H2O | - |
? | |
| L-Fuconate | catabolism of L-fucose | Aureobasidium pullulans | ? | - |
? | |
| L-galactonate | - |
Aureobasidium pullulans | 2-keto-3-deoxy-L-galactonate + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aureobasidium pullulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | less than 50% of maximal activity above and below | Aureobasidium pullulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.4 | - |
Aureobasidium pullulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | less than 50% of maximal activity above and below | Aureobasidium pullulans |
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