Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, pET26b construct p3460, BL21(lambda-DE3) pLysS cells | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, gel filtration and SDS-PAGE | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
three-dimensional protein reconstruction, negative stain electron microscopy, three-dimensional reconstruction, comparative modeling and docking, recombinant cyanide hydratase fibers analyzed by iterative helical real space reconstruction, determination of left-handed D1 S5.4 symmetry with helical rise of 1.36 nm, different arrangement from other microbial nitrilases that display a reduced helical twist, assembly stabilized by two dyadic interactions between dimers across the one-start helical groove, stereoview of the docked model | Neurospora crassa |