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Literature summary for 4.2.1.66 extracted from
- The nitrilase family of CN hydrolyzing enzymes - a comparative study (2003), J. Appl. Microbiol., 95, 1161-1174.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | enzyme induction | Stemphylium loti |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence analysis | Fusarium solani |
| DNA sequence analysis | Microdochium sorghi |
| DNA sequence analysis | Fusarium lateritium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D275E | site-directed mutagenesis, 0-29% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| F170L | site-directed mutagenesis, inactive mutant compared to the wild-type enzyme | Fusarium lateritium |
| K136R | site-directed mutagenesis, 52% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| M302S | site-directed mutagenesis, 22% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| S13A | site-directed mutagenesis, 19-47% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| T12P | site-directed mutagenesis, inactive mutant | Fusarium lateritium |
| T12Q | site-directed mutagenesis, 10-48% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| V281A | site-directed mutagenesis, 8% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000 | Fusarium solani |
| 40000 | - |
x * 40000 | Fusarium oxysporum |
| 40000 | - |
x * 40000 | Microdochium sorghi |
| 40000 | - |
x * 40000 | Fusarium lateritium |
| 40000 | - |
n * 40000 | Leptosphaeria maculans |
| 160000 | - |
- |
Leptosphaeria maculans |
| 300000 | - |
above | Fusarium solani |
| 300000 | - |
above | Fusarium lateritium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium lateritium | - |
- |
- |
| Fusarium oxysporum | - |
- |
- |
| Fusarium oxysporum N-10 | - |
- |
- |
| Fusarium solani | - |
- |
- |
| Fusarium solani IMI 196840 | - |
- |
- |
| Leptosphaeria maculans | - |
- |
- |
| Microdochium sorghi | - |
- |
- |
| Stemphylium loti | - |
pathogen of the cyanogenic plant Lotus corniculatus | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| formamide = cyanide + H2O | reaction mechanism | Fusarium solani | |
| formamide = cyanide + H2O | reaction mechanism | Fusarium oxysporum | |
| formamide = cyanide + H2O | reaction mechanism | Stemphylium loti | |
| formamide = cyanide + H2O | reaction mechanism | Microdochium sorghi | |
| formamide = cyanide + H2O | reaction mechanism | Fusarium lateritium | |
| formamide = cyanide + H2O | reaction mechanism | Leptosphaeria maculans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum | ? | - |
? | |
| acrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum N-10 | ? | - |
? | |
| crotononitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum | ? | - |
? | |
| crotononitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum N-10 | ? | - |
? | |
| HCN + H2O | - |
Fusarium solani | formamide | - |
? | |
| HCN + H2O | - |
Fusarium oxysporum | formamide | - |
? | |
| HCN + H2O | - |
Stemphylium loti | formamide | - |
? | |
| HCN + H2O | - |
Microdochium sorghi | formamide | - |
? | |
| HCN + H2O | - |
Fusarium lateritium | formamide | - |
? | |
| HCN + H2O | - |
Leptosphaeria maculans | formamide | - |
? | |
| HCN + H2O | - |
Fusarium solani IMI196840 | formamide | - |
? | |
| HCN + H2O | - |
Fusarium solani IMI 196840 | formamide | - |
? | |
| HCN + H2O | - |
Fusarium oxysporum N-10 | formamide | - |
? | |
| methacrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum | ? | - |
? | |
| methacrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum N-10 | ? | - |
? | |
| additional information | hydrolysis of a metal-cyano complex, tetracyanonickelate | Fusarium solani | ? | - |
? | |
| additional information | low hydrolytic activity with a metal-cyano complex, tetracyanonickelate, the enzyme also shows nitrilase activity | Fusarium oxysporum | ? | - |
? | |
| additional information | hydrolysis of a metal-cyano complex, tetracyanonickelate | Fusarium solani IMI196840 | ? | - |
? | |
| additional information | hydrolysis of a metal-cyano complex, tetracyanonickelate | Fusarium solani IMI 196840 | ? | - |
? | |
| additional information | low hydrolytic activity with a metal-cyano complex, tetracyanonickelate, the enzyme also shows nitrilase activity | Fusarium oxysporum N-10 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 40000 | Fusarium solani |
| oligomer | x * 40000 | Fusarium oxysporum |
| oligomer | x * 40000 | Microdochium sorghi |
| oligomer | x * 40000 | Fusarium lateritium |
| tetramer | n * 40000 | Leptosphaeria maculans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| formamide hydrolase | - |
Fusarium solani |
| formamide hydrolase | - |
Fusarium oxysporum |
| formamide hydrolase | - |
Stemphylium loti |
| formamide hydrolase | - |
Microdochium sorghi |
| formamide hydrolase | - |
Fusarium lateritium |
| formamide hydrolase | - |
Leptosphaeria maculans |
| More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Fusarium solani |
| More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Fusarium oxysporum |
| More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Stemphylium loti |
| More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Microdochium sorghi |
| More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Fusarium lateritium |
| More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Leptosphaeria maculans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
broad range | Stemphylium loti |
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