Literature summary for 4.2.1.65 extracted from

Yanase, H.; Sakai, T.; Tonomura, K.
Microbial utilization of beta-cyano-L-alanine by Pseudomonas sp. 13 (1982), Agric. Biol. Chem., 46, 2925-2932.

No PubMed abstract available

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General Stability

General Stability	Organism
not stabilized by rapid freezing in liquid nitrogen, storage at 4 or -20¦C with mercaptoethanol, dithiothreitol, beta-cyano-L-alanine or asparagine	Pseudomonas sp.
stabilized as ammonium sulfate precipitate	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	13	-
Pseudomonas sp. 13	-	13	-

Storage Stability

Storage Stability	Organism
-20°C, 10% glycerol, 10-15% loss of activity after 5 days	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-Cyano-L-alanine + H2O	highly specific for beta-cyano-L-alanine	Pseudomonas sp.	L-Asparagine	asparagine is subsequently degraded to aspartic acid and ammonia	?
beta-Cyano-L-alanine + H2O	highly specific for beta-cyano-L-alanine	Pseudomonas sp. 13	L-Asparagine	asparagine is subsequently degraded to aspartic acid and ammonia	?

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Release 2023.1 (January 2023)