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Literature summary for 4.2.1.6 extracted from

  • Szumilo, T.
    Purification and properties of D-galactonate dehydratase from Mycobacterium butyricum (1981), Biochim. Biophys. Acta, 661, 240-246.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CdSO4
-
Mycobacterium butyricum
CuSO4
-
Mycobacterium butyricum
HgCl2
-
Mycobacterium butyricum
iodoacetate
-
Mycobacterium butyricum
NaF
-
Mycobacterium butyricum
PCMB
-
Mycobacterium butyricum
ZnSO4 reactivated by chelating agents Mycobacterium butyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
D-galactonate
-
Mycobacterium butyricum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ highest activity at 0.3 mM MgCl2, Mg2+ or Mn2+ required Mycobacterium butyricum
Mn2+ highest activity at 0.02 mM MnCl2, Mn2+ or Mg2+ required Mycobacterium butyricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
gel filtration Mycobacterium butyricum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactonate Mycobacterium butyricum the enzyme catalyzes the first reaction in the D-galactonate catabolic pathway of non-pathogenic mycobacteria ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium butyricum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycobacterium butyricum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.56
-
-
Mycobacterium butyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactonate the enzyme catalyzes the first reaction in the D-galactonate catabolic pathway of non-pathogenic mycobacteria Mycobacterium butyricum ?
-
?
D-galactonate the enzyme is strictly specific for D-galactonate Mycobacterium butyricum 2-dehydro-3-deoxy-D-galactonate + H2O
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
dithiothreitol increases heat sensitivity Mycobacterium butyricum
70
-
15 min, about 10% loss of activity Mycobacterium butyricum
75
-
10 min, about 60% loss of activity. 15 min, about 90% loss of activity Mycobacterium butyricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8 in 100 mM tris-HCl buffer or 50 mM phosphate buffer Mycobacterium butyricum