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Literature summary for 4.2.1.51 extracted from

  • Zhou, H.; Liao, X.; Wang, T.; Du, G.; Chen, J.
    Enhanced l-phenylalanine biosynthesis by co-expression of pheA(fbr) and aroF(wt) (2010), Biores. Technol., 101, 4151-4156.
    View publication on PubMed
Search for more literature for 4.2.1.51

Application

Application Comment Organism
industry overexpression of pheAfbr and/or wild-type aroF is helpful for the enhancement of the key enzymes in L-phenylalanine biosynthesis, which is an important prerequisite for improved production of L-phenylalanine with engineered strains Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in different engineered Escherichia coli WSH-Z06 strains (pAP-B, pAP-B01, pAP-B02 and pAP-B03) Escherichia coli

Protein Variants

Protein Variants Comment Organism
T326P mutant harboring pheAfbr retains more than 70% of CM and PDT activities even in the presence of 200 mM L-phenylalanine, has potential in overproduction of L-phenylalanine Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-phenylalanine feedback inhibition. PDT of Escherichia coli WSH-Z06 is almost completely inhibited by 10 mM L-phenylalanine (92.9% activity loss). PDT of Escherichia coli WSH-Z06 (pAP-B03) exhibits strong resistance to 200 mM L-phenylalanine, manifested by the high residual activities (10 mM L-phenylalanine with only 7.1% loss of activity) Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43100
-
 x * 43100, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli C7EXK8 the enzyme possesses prephenate dehydratase (EC 4.2.1.51) and chorismate mutase activity (EC 5.4.99.5); K06
-
Escherichia coli K06 C7EXK8 the enzyme possesses prephenate dehydratase (EC 4.2.1.51) and chorismate mutase activity (EC 5.4.99.5); K06
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3100
-
 strain WSH-Z06 (pAP-B) Escherichia coli
18000
-
 strain WSH-Z06 (pAP-B03) Escherichia coli
18600
-
 strain WSH-Z06 (pAP-B01) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prephenate
-
 Escherichia coli phenylpyruvate + H2O + CO2
-
 ?
prephenate
-
 Escherichia coli K06 phenylpyruvate + H2O + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 43100, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
chorismate mutase-prephenate dehydratase the enzyme also possesses chorismate mutase activity (EC 5.4.99.5) Escherichia coli
CM–PDT the enzyme also possesses chorismate mutase activity (EC 5.4.99.5) Escherichia coli
PheA
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction use of a T326P mutation in the regulation domain of pheA (pheAfbr) for L-phenylalanine production. Overexpression of pheAfbr and/or wild-type aroF is helpful for the enhancement of the key enzymes in L-phenylalanine biosynthesis Escherichia coli