Cloned (Comment) | Organism |
---|---|
the recombinant C-terminally His6-tagged protein is expressed in Escherichia coli | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
the enzyme is crystallized in several conditions, with the best crystals obtained with 0.04 M dipotassium hydrogen phosphate, 16.00 % (w/v) polyethylene glycol 8000 and 22.0% (v/v) glycerol. X ray diffraction data are collected to 2.61 A resolution. The crystals belong to the monoclinic space group P21, with unit cell parameters a = 85.12, b = 137.63, c = 117.69 A, beta = 94.69° and are suitable for molecular replacement phasing | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4D9S6 | - |
- |
Trypanosoma cruzi CL Brener | Q4D9S6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in histidine degradation and is essential for the catabolic conversion of urocanate to 4-imidazolone-5-propionate, which finally yields glutamate and further glutamine and tricarboxylic acid cycle intemediates such as 2-oxo-pentanedioate | Trypanosoma cruzi |