Cloned (Comment) | Organism |
---|---|
His6-GMD expressed from the pQE-30-gmd vector | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-mannose | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01402 | - |
GDP-mannose | pH 7.5, 37°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q51366 | - |
- |
Purification (Comment) | Organism |
---|---|
by ultrasonication, centrifugation, on Ni2+-nitrilotriacetic acid resin and by anion exchange chromatography, to greater than 95% purity | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-80°C, 25% glycerol, 1 year | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose | i.e., GDP-6-deoxy-D-lyxo-hexos-4-ulose | Pseudomonas aeruginosa | GDP-D-rhamnose | - |
? | |
GDP-mannose | is a bifunctional 4,6-dehydratase, and a stereospecific 4-reductase. A conserved amino acid side chain (Arg185) may be crucial for orienting substrate and cofactor | Pseudomonas aeruginosa | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDP-D-mannose-4,6-dehydratase | - |
Pseudomonas aeruginosa |
GMD | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
His6-GMD loses activity slowly in the course of enzyme-substrate incubations, particularly at 37°C, but 4,6-dehydratase activity is still detectable after incubation for 16 h at 25°C | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.82 | - |
GDP-mannose | pH 7.5, 37°C | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | addition of NADPH results in the gradual, His6-GMD-dependent reduction of GDP-6-deoxy-D-lyxo-hexos-4-ulose to GDP-D-rhamnose | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002859 | - |
GDP-mannose | pH 7.5, 37°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
630 | - |
GDP-mannose | pH 7.5, 37°C | Pseudomonas aeruginosa |