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Literature summary for 4.2.1.47 extracted from
- Biochemical characterization of GDP-L-fucose de novo synthesis pathway in fungus Mortierella alpina (2010), Biochem. Biophys. Res. Commun., 391, 1663-1669.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | enhances GMD activity by 2.3fold | Mortierella alpina |  |
| DTT | enhances GMD activity by 2.3fold | Mortierella alpina | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pET28a+ to construct pLW1534, expressed in Escherichia coli BL21 as His-tagged fusion protein | Mortierella alpina |
| recombinant expression of His-tagged GMD in Escherichia coli | Mortierella alpina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, strong inhibition; strong inhibition at 5 mM | Mortierella alpina | |
| Cu2+ | 5 mM, strong inhibition; strong inhibition at 5 mM | Mortierella alpina | |
| Fe2+ | 5 mM, strong inhibition; strong inhibition at 5 mM | Mortierella alpina | |
| additional information | is not affected by Ca2+ and Mn2+ | Mortierella alpina |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.77 | - |
GDP-mannose | pH 8.0, 37°C | Mortierella alpina | |
| 0.77 | - |
GDP-mannose | recombinant enzyme, pH 8.0, 37°C | Mortierella alpina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, activity is significantly enhanced | Mortierella alpina | |
| Mg2+ | activates at 5 mM | Mortierella alpina | |
| additional information | divalent cations are not required for GMD activity | Mortierella alpina | |
| additional information | GMD activity is not affected by Ca2+ at 5 mM | Mortierella alpina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41200 | - |
3 * 45000, recombinant His-tagged GMD, SDS-PAGE, 3 * 41200, about, sequence calculation | Mortierella alpina |
| 45000 | - |
3 * 45000, SDS-PAGE | Mortierella alpina |
| 45000 | - |
3 * 45000, recombinant His-tagged GMD, SDS-PAGE, 3 * 41200, about, sequence calculation | Mortierella alpina |
| 140000 | - |
gel filtration | Mortierella alpina |
| 140000 | - |
recombinant His-tagged GMD, native PAGE | Mortierella alpina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose | Mortierella alpina | - |
GDP-4-keto-6-deoxy mannose + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mortierella alpina | D3JU53 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged GMD from Escherichia coli by nickel affinity chromatography | Mortierella alpina |
| to near homogeneity by nickel ion affinity chromatography | Mortierella alpina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose | - |
Mortierella alpina | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
| GDP-mannose | - |
Mortierella alpina | GDP-4-keto-6-deoxy mannose + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 45000, SDS-PAGE | Mortierella alpina |
| trimer | 3 * 45000, recombinant His-tagged GMD, SDS-PAGE, 3 * 41200, about, sequence calculation | Mortierella alpina |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDP-D-mannose 4,6-dehydratase | - |
Mortierella alpina |
| GMD | - |
Mortierella alpina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mortierella alpina |
| 37 | - |
recombinant enzyme | Mortierella alpina |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | 25°C: about 60% of maximal activity, 55°C: about 70% of maximal activity | Mortierella alpina |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.96 | - |
GDP-mannose | pH 8.0, 37°C | Mortierella alpina | |
| 5.96 | - |
GDP-mannose | recombinant enzyme, pH 8.0, 37°C | Mortierella alpina | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Mortierella alpina |
| 9 | - |
recombinant enzyme | Mortierella alpina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | pH 5: 45% of maximal activity, pH 11: 75% of maximal activity | Mortierella alpina |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Mortierella alpina | |
| NADP+ | - |
Mortierella alpina | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | GMD catalyzes the first step in the de novo synthesis pathways of GDP-L-fucose, overview | Mortierella alpina |
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