Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K161A | 3000fold reduced kcat | Escherichia coli |
T133V | 3000fold reduced kcat | Escherichia coli |
Y157F | 3000fold reduced kcat | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-fucose | competitive inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
GDP-mannose | pH 7, 37°C, T133V mutant | Escherichia coli | |
0.28 | - |
GDP-mannose | pH 7, 37°C, wild-type | Escherichia coli | |
0.501 | - |
GDP-mannose | pH 7, 37°C, K161A mutant | Escherichia coli | |
1.02 | - |
GDP-mannose | pH 7, 37°C, Y157F mutant | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose | - |
Escherichia coli | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, gel filtration, equilibrium ultracentrifugation experiments | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
GDP-mannose | pH 7, 37°C, T133V mutant | Escherichia coli | |
0.0019 | - |
GDP-mannose | pH 7, 37°C, Y157F mutant | Escherichia coli | |
0.0053 | - |
GDP-mannose | pH 7, 37°C, K161A mutant | Escherichia coli | |
5 | - |
GDP-mannose | pH 7, 37°C, wild-type | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | one NADP+ per monomer | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0789 | - |
GDP-fucose | pH 7, 37°C | Escherichia coli |