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Literature summary for 4.2.1.46 extracted from

  • Gilbert, J.M.; Matsuhashi, M.; Strominger, J.L.
    Thymidine diphosphate 4-acetamido-4,6-dideoxyhexoses. II. Purification and properties of thymidine diphosphate D-glucose oxidoreductase (1965), J. Biol. Chem., 240, 1305-1308.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuriphenylsulfonate partial reactivation with cysteine and NAD+ Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 dTDPglucose
-
 Escherichia coli
0.1 0.2 NAD+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-glucose Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 dTDPglucose Escherichia coli

Storage Stability

Storage Stability Organism
10% loss of activity during storage at 0¦C for 1 week Escherichia coli
stable for several weeks at -20¦C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-glucose
-
 Escherichia coli dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
dTDP-glucose
-
 Escherichia coli ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 dTDPglucose Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli