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Literature summary for 4.2.1.46 extracted from
- Biological mechanisms involved in the formation of deoxysugars. V. Isolation and characterization of thymidine diphosphate D-glucose oxidoreductase from Escherichia coli B (1969), J. Biol. Chem., 244, 3430-3437.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTDP-6-deoxy-D-glucose | - |
Escherichia coli |  |
| p-chloromercuriphenylsulfonate | partial reactivation with cysteine and NAD+ | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.071 | - |
dTDPglucose | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
sedimentation equilibrium centrifugation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-glucose | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.3 | - |
dTDPglucose | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable at -12¦C, 10% loss of activity during storage at room temperature for 1 h, loss of activity upon repeated freezing and thawing | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-glucose | - |
Escherichia coli | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
| dTDP-glucose | - |
Escherichia coli | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
dTDPglucose | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | 1 mol of bound NAD+ per mol of enzyme | Escherichia coli | |
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