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Literature summary for 4.2.1.44 extracted from

  • Berman, T.; Magasanik, B.
    The pathway of myo-inositol degradation in Aerobacter aerogenes. Dehydrogenation and dehydration (1966), J. Biol. Chem., 241, 800-806.
    View publication on PubMed
Search for more literature for 4.2.1.44

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibition can be alleviated by either Mn2+ or Co2+ Klebsiella aerogenes
p-hydroxymercuribenzoate on addition of glutathione or mercaptoethanol the activity is regained Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 2-keto-myo-inositol
-
 Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ increases activity Klebsiella aerogenes
Mn2+ increases activity Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone Klebsiella aerogenes pathway of myo-inositol degradation (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Oxidation Stability

Oxidation Stability Organism
unstable when exposed to oxygen Klebsiella aerogenes

Purification (Commentary)

Purification (Comment) Organism
partial Klebsiella aerogenes

Reaction

Reaction Comment Organism Reaction ID
2,4,6/3,5-Pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O mechanism Klebsiella aerogenes
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
270
-
-
 Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-10°C, nitrogen atmosphere, for 1 month, about 50% loss of activity Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone
-
 Klebsiella aerogenes (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
-
 ?
(2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone pathway of myo-inositol degradation Klebsiella aerogenes (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
-
 ?
L-2-Keto-epi-inositol
-
 Klebsiella aerogenes ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.2 2-keto-myo-inositol Klebsiella aerogenes