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Literature summary for 4.2.1.42 extracted from

  • Easton, N.M.; Aboushawareb, S.A.E.; Bearne, S.L.
    A continuous assay for L-talarate/galactarate dehydratase using circular dichroism (2018), Anal. Biochem., 544, 80-86 .
    View publication on PubMed

Application

Application Comment Organism
analysis a continuous assay for L-talarate/galactarate dehydratase is develped using circular dichroism. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes Salmonella enterica subsp. enterica serovar Typhimurium
analysis a convenient, direct continuous circular dichroism-based assay is developed for following the L-talarate/galactarate dehydratase-catalyzed conversion of meso-galactarate to 5-dehydro-4-deoxy-D-glucarate. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

Cloned (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
Tartronate competitive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
meso-galactarate pH 8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.38
-
galactarate pH 8.0, 25°C, His6-tagged recombinant enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q8ZL58
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactarate
-
Salmonella enterica subsp. enterica serovar Typhimurium (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O
-
?
galactarate
-
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O
-
?
L-talarate
-
Salmonella enterica subsp. enterica serovar Typhimurium 5-dehydro-4-deoxy-D-glucarate + H2O
-
?
L-talarate
-
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 5-dehydro-4-deoxy-D-glucarate + H2O
-
?
meso-galactarate
-
Salmonella enterica subsp. enterica serovar Typhimurium 5-dehydro-4-deoxy-D-glucarate + H2O
-
?
meso-galactarate
-
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 5-dehydro-4-deoxy-D-glucarate + H2O
-
?

Synonyms

Synonyms Comment Organism
L-talarate/galactarate dehydratase
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.8
-
meso-galactarate pH 8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
4.8
-
galactarate pH 8.0, 25°C, His6-tagged recombinant enzyme Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
10.7
-
Tartronate pH 8.0, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
10.7
-
Tartronate pH 8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13
-
meso-galactarate pH 8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
13
-
galactarate pH 8.0, 25°C, His6-tagged recombinant enzyme Salmonella enterica subsp. enterica serovar Typhimurium