Application | Comment | Organism |
---|---|---|
analysis | a continuous assay for L-talarate/galactarate dehydratase is develped using circular dichroism. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
analysis | a convenient, direct continuous circular dichroism-based assay is developed for following the L-talarate/galactarate dehydratase-catalyzed conversion of meso-galactarate to 5-dehydro-4-deoxy-D-glucarate. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tartronate | competitive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.38 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZL58 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O | - |
? | |
galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O | - |
? | |
L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
meso-galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
meso-galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-talarate/galactarate dehydratase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.8 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
Tartronate | pH 8.0, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
10.7 | - |
Tartronate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
13 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |