Literature summary for 4.2.1.40 extracted from

Aghaie, A.; Lechaplais, C.; Sirven, P.; Tricot, S.; Besnard-Gonnet, M.; Muselet, D.; de Berardinis, V.; Kreimeyer, A.; Gyapay, G.; Salanoubat, M.; Perret, A.
New Insights into the Alternative D-Glucarate Degradation Pathway (2008), J. Biol. Chem., 283, 15638-15646.

Search for more literature for 4.2.1.40

Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells	Acinetobacter baylyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.101	-	D-glucarate	-	Acinetobacter baylyi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34960	-	calculated	Acinetobacter baylyi
38000	-	determined by SDS-PAGE	Acinetobacter baylyi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucarate	Acinetobacter baylyi	-	5-dehydro-4-deoxy-D-glucarate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baylyi	Q6FFQ2	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column	Acinetobacter baylyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucarate	-	Acinetobacter baylyi	5-dehydro-4-deoxy-D-glucarate + H2O	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Acinetobacter baylyi

Synonyms

Synonyms	Comment	Organism
D-Glucarate dehydratase	-	Acinetobacter baylyi
GDH	-	Acinetobacter baylyi
glucarate dehydratase	-	Acinetobacter baylyi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	activity assay	Acinetobacter baylyi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.39	-	D-glucarate	-	Acinetobacter baylyi

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Release 2023.1 (January 2023)