KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-galactonate | - |
Picrophilus torridus | |
2.5 | - |
gluconate | - |
Picrophilus torridus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | is required | Picrophilus torridus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
8 * 44000, SDS-PAGE | Picrophilus torridus |
340000 | - |
gel filtration | Picrophilus torridus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6L1T2 | - |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation and gel filtration, 190fold to apparent homogeneity | Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactonate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
gluconate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
additional information | xylonate (10 mM) is not utilized | Picrophilus torridus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 44000, SDS-PAGE | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Picrophilus torridus |
gluconate dehydratase | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life is about 15 min | Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
no loss of activity upon incubation for 2 h | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Picrophilus torridus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.1 | 50% of activity at pH 8.1 and at pH 4.5 | Picrophilus torridus |