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Literature summary for 4.2.1.39 extracted from

  • Kim, S.; Lee, S.B.
    Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners (2008), Protein Expr. Purif., 62, 116-119.
    View publication on PubMed
Search for more literature for 4.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
vectors for constructing different fusion proteins are used, pET-21a, 6xHis, pET-32a, thioredoxin, Trx, pET-42a, glutathione-S-transferase, GST, and pET-43.1a, N-utilization substance A, NusA Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44730
-
 rGNAD, His-tagged, calculated Saccharolobus solfataricus
45000
-
 rGNAD, His-tagged, determined by SDS-PAGE Saccharolobus solfataricus
56000
-
 rGNAD, Trx-fusion, determined by SDS-PAGE Saccharolobus solfataricus
71000
-
 rGNAD, GST-fusion, determined by SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-gluconate Saccharolobus solfataricus
-
 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
D-gluconate dehydratase
-
 Saccharolobus solfataricus
gluconate dehydratase
-
 Saccharolobus solfataricus
GNAD
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 standard assay condition Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 standard assay condition Saccharolobus solfataricus