General Stability | Organism |
---|---|
rather labile | Alcaligenes sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Alcaligenes sp. | |
Co2+ | - |
Alcaligenes sp. | |
EDTA | 70% inhibition at 0.1 mM | Alcaligenes sp. | |
EDTA | - |
Clostridium pasteurianum | |
Ni2+ | - |
Alcaligenes sp. | |
p-chloromercuribenzoate | 30% inhibition at 0.01 mM | Alcaligenes sp. | |
p-chloromercuribenzoate | - |
Clostridium pasteurianum | |
Zn2+ | - |
Alcaligenes sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.8 | - |
D-gluconate | - |
Alcaligenes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace | Clostridium pasteurianum | |
Mg2+ | Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace | Alcaligenes sp. | |
Mg2+ | 100% activation at 1 mM | Alcaligenes sp. | |
Mn2+ | 50% activation at 0.1 mM, 30% activation at 1 mM | Alcaligenes sp. | |
Mn2+ | Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace | Clostridium pasteurianum | |
Mn2+ | Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace | Alcaligenes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
thin-layer chromatography | Alcaligenes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Alcaligenes sp. | - |
properties are conspiciously different from those of Clostridium pasteurianum | - |
Alcaligenes sp. M250 | - |
properties are conspiciously different from those of Clostridium pasteurianum | - |
Aspergillus niger | - |
- |
- |
Cereibacter sphaeroides | - |
i.e. Rhodopseudomonas sphaeroides | - |
Clostridium pasteurianum | - |
- |
- |
Clostridium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.2 | - |
- |
Alcaligenes sp. |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months | Clostridium pasteurianum |
-20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months | Alcaligenes sp. |
0°C, Tris-HCl buffer, pH 8.0-8.8, MgCl2, sodium-D-gluconate, 50% loss of activity after 7 days | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fuconate | 15% of activity with D-gluconate | Alcaligenes sp. | ? | - |
? | |
D-fuconate | 15% of activity with D-gluconate | Alcaligenes sp. M250 | ? | - |
? | |
D-gluconate | - |
Clostridium pasteurianum | 2-dehydro-3-deoxy-D-gluconate | - |
? | |
D-gluconate | - |
Alcaligenes sp. | 2-dehydro-3-deoxy-D-gluconate | - |
? | |
D-gluconate | - |
Alcaligenes sp. M250 | 2-dehydro-3-deoxy-D-gluconate | - |
? | |
D-xylonate | 89% of activity with D-gluconate | Alcaligenes sp. | ? | - |
? | |
D-xylonate | 89% of activity with D-gluconate | Alcaligenes sp. M250 | ? | - |
? | |
additional information | - |
Clostridium pasteurianum | ? | - |
? | |
additional information | specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested | Alcaligenes sp. | ? | - |
? | |
additional information | specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested | Alcaligenes sp. M250 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme assay at | Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.8 | - |
Alcaligenes sp. |