Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.39 extracted from

  • Bender, R.; Gottschalk, G.
    Purification and properties of D-gluconate dehydratase from Clostridium pasteurianum (1973), Eur. J. Biochem., 40, 309-321.
    View publication on PubMed
Search for more literature for 4.2.1.39

Activating Compound

Activating Compound Comment Organism Structure
additional information several metabolites tested, but without effect Clostridium pasteurianum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Clostridium pasteurianum
dithiothreitol
-
 Clostridium pasteurianum
EDTA
-
 Clostridium pasteurianum
L-cysteine
-
 Clostridium pasteurianum
p-chloromercuribenzoate
-
 Clostridium pasteurianum
sulfhydryl compounds
-
 Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5
-
 D-gluconate
-
 Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
Fe2+
-
 Clostridium pasteurianum
Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 2 * 64000, SDS-PAGE Clostridium pasteurianum
118000
-
 sucrose density gradient centrifugation Clostridium pasteurianum
131000
-
 sedimentation equilibrium, gel filtration Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-gluconate Clostridium pasteurianum first enzyme in modified Entner-Doudoroff pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Oxidation Stability

Oxidation Stability Organism
inactivation by O2, activity restored by sulfhydryl compounds in the presence of Fe2+ Clostridium pasteurianum

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
132
-
-
 Clostridium pasteurianum

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months Clostridium pasteurianum
4°C, Tris-HCl buffer, pH 7.1, reactivated enzyme, at least 3 weeks under anaerobic conditions Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate
-
 Clostridium pasteurianum 2-dehydro-3-deoxy-D-gluconate
-
 ?
D-gluconate first enzyme in modified Entner-Doudoroff pathway Clostridium pasteurianum ?
-
 ?
additional information only substrate is D-gluconate, not: D-galactonate, D-galactarate, D-galacturonate, 6-phospho-D-gluconate, D-arabonate, D-xylonate, L-mannonate, D-glucoheptonate Clostridium pasteurianum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Clostridium pasteurianum
More number of sulfhydryl groups Clostridium pasteurianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 enzyme assay at Clostridium pasteurianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 reactivation possible after 45 min exposure which leads to complete loss of activity, higher temperatures cause irreversible heat inactivation Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.8
-
 Clostridium pasteurianum

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Clostridium pasteurianum