Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | 0.15 mM, complete inhibition. 80% protection by 5 mM S-citramalate | Aerobic bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
mesaconate | - |
Aerobic bacterium | |
0.11 | - |
fumarate | - |
Aerobic bacterium | |
0.45 | - |
S-citramalate | - |
Aerobic bacterium | |
0.56 | - |
S-malate | - |
Aerobic bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Aerobic bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aerobic bacterium | constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aerobic bacterium | - |
- |
- |
Oxidation Stability | Organism |
---|---|
4°C, 90% loss of activity after 24 h in air. Reactivation by Fe2+ and 2-mercaptoethanol | Aerobic bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Aerobic bacterium |
Storage Stability | Organism |
---|---|
-20°C, in presence of 20% (NH4)2SO4 or 10% glycerol in air, about 80-90% can be reactivated even after several months | Aerobic bacterium |
4°C, 70% loss of activity after 1 week under N2 | Aerobic bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L)-citramalate | - |
Aerobic bacterium | mesaconate + H2O | - |
? | |
(S)-malate | - |
Aerobic bacterium | fumarate + H2O | - |
? | |
Citramalate + H2O | - |
Aerobic bacterium | Mesaconate | - |
? | |
fumarate + H2O | - |
Aerobic bacterium | L-malate | - |
? | |
mesaconate | - |
Aerobic bacterium | citramalate + H2O | - |
? | |
additional information | constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate | Aerobic bacterium | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
with mesaconate as substrate | Aerobic bacterium |
8.7 | - |
with fumarate as substrate | Aerobic bacterium |
9 | - |
S-malate as substrate | Aerobic bacterium |
9.1 | - |
with S-citramalate as substrate | Aerobic bacterium |