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Literature summary for 4.2.1.33 extracted from
- Structural characterization and comparison of the large subunits of IPM isomerase and homoaconitase from Methanococcus jannaschii (2014), Acta Crystallogr. Sect. D, 70, 922-931.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) Star cells | Methanocaldococcus jannaschii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting-drop vapour-diffusion methodand hanging-drop vapour diffusion at 20°C, structures of oxidized and reduced forms of the large subunit of isopropylmalate isomerase (ox-MJ0499 and red-MJ0499, respectively) are reported at 1.8 and 2.7 A resolution, respectively. Significant large conformational changes are observed in the active site of red-MJ0499 when compared with ox-MJ0499 | Methanocaldococcus jannaschii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron-sulfur cluster | the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site | Methanocaldococcus jannaschii |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | P81291 | large subunit of 3-isopropylmalate dehydratase | - |
| Methanocaldococcus jannaschii DSM 2661 | P81291 | large subunit of 3-isopropylmalate dehydratase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| isopropylmalate isomerase | - |
Methanocaldococcus jannaschii |
| leuC | - |
Methanocaldococcus jannaschii |
| MJ0499 | gene name, large subunit of 3-isopropylmalate dehydratase | Methanocaldococcus jannaschii |
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