Literature summary for 4.2.1.30 extracted from

Sankaranarayanan, M.; Seol, E.; Kim, Y.; Chauhan, A.S.; Park, S.
Measurement of crude-cell-extract glycerol dehydratase activity in recombinant Escherichia coli using coupled-enzyme reactions (2017), J. Ind. Microbiol. Biotechnol., 44, 477-488 .

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Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	Q59476 AND O08505 AND Q59475	Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	a reliable, enzyme-coupled assay for measuring glycerol dehydratase activity in crude-cell extract is developed using 1,2-propanediol as the substrate. In the assay, 1,2-propanediol is converted to propionaldehyde, which is quickly converted to 1-propionic acid by aldehyde dehydrogenase (with the production of NADH) or to 1-propanol by yeast alcohol dehydrogenase (with the consumption of NADH). The change in NADH concentration, as monitored at 340 nm spectrophotometrically, manifested as a straight line for 3 min, from which the glycerol dehydratase activity can be determined. The assay method should prove to be applicable to recombinant strains developed for the production of 3-hydroxypropionic acid and/or 1,3-propanediol from glycerol	Klebsiella pneumoniae

Synonyms

Synonyms	Comment	Organism
GDHt	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)