Literature summary for 4.2.1.30 extracted from

Hong, E.; Kim, J.; Ha, S.; Ryu, Y.
Improved 1,3-propanediol production by Escherichia coli from glycerol due to co-expression of glycerol dehydratase reactivation factors and succinate addition (2015), Biotechnol. Bioprocess Eng., 20, 849-855 .

Search for more literature for 4.2.1.30

Application

Application	Comment	Organism
synthesis	Escherichia coli is engineered to produce 1,3-propanediol from glycerol, an inexpensive carbon source. This is done by introducing a synthetic 1,3-propanediol production pathway in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli. When 10 mM succinate is added to the medium, the titer of 1,3-propanediol and the glycerol consumption increase to 9.9 and 23.84 g/l, respectively. In addition, the ratio of NADH to NAD+ increases by 43%. Succinate addition is a promising route for the biotechnological production of NADH-dependent microbial metabolites	Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	Q59476 AND O08505 AND Q59475	Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycerol	-	Klebsiella pneumoniae	3-hydroxypropanal + H2O	-	?

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Release 2023.1 (January 2023)