Literature summary for 4.2.1.30 extracted from

Jiang, W.; Li, W.; Hong, Y.; Wang, S.; Fang, B.
Cloning, expression, mutagenesis library construction of glycerol dehydratase, and binding mode simulation of its reactivase with ligands (2016), Appl. Biochem. Biotechnol., 178, 739-752 .

Search for more literature for 4.2.1.30

Application

Application	Comment	Organism
synthesis	glycerol dehydratase is a key and rate-limiting enzyme in the bioconversion process of glycerol to 3-hydroxypropionaldehyde, which is further reduced to 1,3-propanediol by 1,3-propanediol dehydrogenase. 3-Hydroxypropionaldehyde is an important chemical industry material, being a potent bacterial inhibitor and precursor in the production of numerous industrial chemicals, such as 1,3-propanediol	Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
the enzymes of pET-32a-gldABC (for cellular expression) and pET-22b-gldABC (for extracellular expression) are 6.8 and 3.2 U/mg, about six and three times that of the wild type enzyme, respectively. The empty expression plasmid pET-32a-gldAC containing no gldB is under construction to ligate a mutagenesis fragment of the gldB to construct a mutagenesis library	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	Q59476 AND O08505 AND Q59475	Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit	-
Klebsiella pneumoniae DSM 2026	Q59476 AND O08505 AND Q59475	Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit	-

Synonyms

Synonyms	Comment	Organism
GDHt	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)