Literature summary for 4.2.1.30 extracted from

Lim, H.G.; Noh, M.H.; Jeong, J.H.; Park, S.; Jung, G.Y.
Optimum rebalancing of the 3-hydroxypropionic acid production pathway from glycerol in Escherichia coli (2016), ACS Synth. Biol., 5, 1247-1255 .

Search for more literature for 4.2.1.30

Application

Application	Comment	Organism
synthesis	synthesis of 3-hydroxypropionic acid. UTR engineering is used to maximally increase the activities of glycerol dehydratase and aldehyde dehydrogenase for the high conversion of glycerol to 3-hydroxypropionic acid. Thereafter, the activity of glycerol dehydratase is precisely controlled to avoid the accumulation of 3-hydroxypropionaldehyde by varying expression of dhaB1, a gene encoding a main subunit of glycerol dehydratase. The optimally balanced Escherichia coli HGL_DBK4 shows a substantially enhanced 3-hydroxypropionic acid titer and productivity compared with the parental strain. The yield on glycerol is 0.97 g 3-hydroxypropionic acid/g glycerol, in a fed-batch experiment	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	Q59476 AND O08505 AND Q59475	Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit	-

Synonyms

Synonyms	Comment	Organism
GDHt	-	Klebsiella pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
coenzyme B12	dependent on	Klebsiella pneumoniae

General Information

General Information	Comment	Organism
metabolism	3-hydroxypropionic acid production pathway from glycerol	Klebsiella pneumoniae

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Release 2023.1 (January 2023)