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Literature summary for 4.2.1.3 extracted from

  • Eprintsev, A.T.; Semenova, E.V.; Popov, V.N.
    Induction of aconitate hydratase in hepatocytes of starving rats (2002), Biochemistry (Moscow), 67, 795-801.
    View publication on PubMed
Search for more literature for 4.2.1.3

Activating Compound

Activating Compound Comment Organism Structure
cysteine enzyme activity increases 2-4fold in presence of both Fe2+ and cysteine. 0.1-1 mM Fe2+ and 0.05-0.5 mM cysteine Rattus rattus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 1-phosphate
-
 Rattus rattus
D-glucose 6-phosphate
-
 Rattus rattus
fumarate
-
 Rattus rattus
glyoxylate
-
 Rattus rattus
H2O2 2.3 mM, 90-95% inhibition Rattus rattus
malate
-
 Rattus rattus
oxaloacetate
-
 Rattus rattus
succinate
-
 Rattus rattus
trans-aconitate
-
 Rattus rattus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 79% cytosolic enzyme and 21% mitochondrial enzyme for control animals, 83% cytosolic enzyme and 17% mitochondrial enzyme for starving animals Rattus rattus 5829
-
mitochondrion 79% cytosolic enzyme and 21% mitochondrial enzyme for control animals, 83% cytosolic enzyme and 17% mitochondrial enzyme for starving animals Rattus rattus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enzyme activity increases 2-4fold in presence of both Fe2+ and cysteine. 0.1-1 mM Fe2+ and 0.05-0.5 mM cysteine Rattus rattus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, mitochondrial enzyme, SDS-PAGE Rattus rattus
45000
-
 2 * 45000, cytosolic enzyme, SDS-PAGE Rattus rattus
84000
-
 mitochondrial enzyme, gel filtration Rattus rattus
96000
-
 cytosolic enzyme, gel filtration Rattus rattus

Organism

Organism UniProt Comment Textmining
Rattus rattus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus rattus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.13
-
 mitochondrial enzyme Rattus rattus
11.1
-
 cytosolic enzyme Rattus rattus

Storage Stability

Storage Stability Organism
4°C, activity of the highly purified cytosolic enzyme decreases by 15-20% during the first 24 h of storage and then by 5% every 24 h, the mitochondrial enzyme decreases 35-40% during the first 24 h and then by 15% every 24 h Rattus rattus

Subunits

Subunits Comment Organism
dimer 2 * 41000, mitochondrial enzyme, SDS-PAGE Rattus rattus
dimer 2 * 45000, cytosolic enzyme, SDS-PAGE Rattus rattus

Synonyms

Synonyms Comment Organism
AH
-
 Rattus rattus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
 Rattus rattus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8
-
 Rattus rattus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.14
-
 glyoxylate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
0.33
-
 succinate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
0.4
-
 fumarate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
0.42
-
 glyoxylate cytosolic enzyme, with isocitrate as substrate Rattus rattus
0.51
-
 fumarate cytosolic enzyme, with isocitrate as substrate Rattus rattus
0.61
-
 succinate cytosolic enzyme, with isocitrate as substrate Rattus rattus
0.81
-
 D-glucose 1-phosphate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
1.03
-
 oxaloacetate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
1.14
-
 D-glucose 6-phosphate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
1.37
-
 trans-aconitate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
1.81
-
 malate cytosolic enzyme, with isocitrate as substrate Rattus rattus
1.9
-
 D-glucose 1-phosphate cytosolic enzyme, with isocitrate as substrate Rattus rattus
2.35
-
 D-glucose 6-phosphate cytosolic enzyme, with isocitrate as substrate Rattus rattus
2.8
-
 oxaloacetate cytosolic enzyme, with isocitrate as substrate Rattus rattus
2.94
-
 malate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
3.41
-
 trans-aconitate cytosolic enzyme, with isocitrate as substrate Rattus rattus