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Literature summary for 4.2.1.3 extracted from
- Oxidation of propionate to pyruvate in Escherichia coli: Involvement of methylcitrate dehydratase and aconitase (2002), Eur. J. Biochem., 269, 6184-6194.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Escherichia coli |  |
| EDTA | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
(2R,3S)-2-methylisocitrate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
x * 94000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Z)-2-methylaconitate + H2O | Escherichia coli | enzyme is involved in pathway of oxidation of propionate to pyruvate | (2R,3S)-2-methylisocitrate | - |
r | |
| (Z)-2-methylaconitate + H2O | Escherichia coli W3350 | enzyme is involved in pathway of oxidation of propionate to pyruvate | (2R,3S)-2-methylisocitrate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P36683 | - |
- |
| Escherichia coli W3350 | P36683 | - |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| rapid inactivation by exposure to air | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.9 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3S)-2-methylisocitrate | enzyme is involved in pathway of oxidation of propionate to pyruvate | Escherichia coli | (Z)-2-methylaconitate + H2O | - |
? | |
| (2R,3S)-2-methylisocitrate | enzyme is involved in pathway of oxidation of propionate to pyruvate | Escherichia coli W3350 | (Z)-2-methylaconitate + H2O | - |
? | |
| (2R,3S)-2-methylisocitrate | - |
Escherichia coli | (Z)-2-methyl-aconitate + H2O | - |
r | |
| (2R,3S)-2-methylisocitrate | - |
Escherichia coli W3350 | (Z)-2-methyl-aconitate + H2O | - |
r | |
| (Z)-2-methylaconitate + H2O | enzyme is involved in pathway of oxidation of propionate to pyruvate | Escherichia coli | (2R,3S)-2-methylisocitrate | - |
r | |
| (Z)-2-methylaconitate + H2O | enzyme is involved in pathway of oxidation of propionate to pyruvate | Escherichia coli W3350 | (2R,3S)-2-methylisocitrate | - |
r | |
| additional information | no detectable activity with (2S,3S)-methylcitrate | Escherichia coli | ? | - |
? | |
| additional information | no detectable activity with (2S,3S)-methylcitrate | Escherichia coli W3350 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 94000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcnB | - |
Escherichia coli |
| aconitase B | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | theoretical value | - |
5.24 |
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