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Literature summary for 4.2.1.28 extracted from

  • Ghiaci, P.; Norbeck, J.; Larsson, C.
    2-Butanol and butanone production in Saccharomyces cerevisiae through combination of a B12 dependent dehydratase and a secondary alcohol dehydrogenase using a TEV-based expression system (2014), PLoS ONE, 9, e102774 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis production of 2-butanol, using a TEV-protease based expression system to achieve equimolar expression of the B12-dependent dehydratase subunit and use of NADH-dependent secondary alcohol dehydrogenase Sadh from Gordonia sp. able to catalyze the subsequent conversion of butanone to 2-butanol. A final concentration of 460.2 mg/l 2-butanol and 260.1 mg/l of butanone is found. Availability of NADH is achieved by growing cells lacking the GPD1 and GPD2 isogenes under anaerobic conditions Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Synonyms

Synonyms Comment Organism
PduCDEGH
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Saccharomyces cerevisiae