Application | Comment | Organism |
---|---|---|
synthesis | production of 2-butanol, using a TEV-protease based expression system to achieve equimolar expression of the B12-dependent dehydratase subunit and use of NADH-dependent secondary alcohol dehydrogenase Sadh from Gordonia sp. able to catalyze the subsequent conversion of butanone to 2-butanol. A final concentration of 460.2 mg/l 2-butanol and 260.1 mg/l of butanone is found. Availability of NADH is achieved by growing cells lacking the GPD1 and GPD2 isogenes under anaerobic conditions | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
PduCDEGH | - |
Saccharomyces cerevisiae |