Crystallization (Comment) | Organism |
---|---|
structures in the absence and presence of substrates at resolutions of 1.55-2.05 A | Klebsiella oxytoca |
Protein Variants | Comment | Organism |
---|---|---|
S224A | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 5fold reduction in kcat/Km | Klebsiella oxytoca |
S224C | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. About 100fold reduction in kcat/Km | Klebsiella oxytoca |
S224N | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 100fold reduction in kcat/Km | Klebsiella oxytoca |
T172A | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 100fold reduction in kcat/Km | Klebsiella oxytoca |
T172S | mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 10fold reduction in kcat/Km | Klebsiella oxytoca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
Propane-1,2-diol | mutant T172A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
0.15 | - |
Propane-1,2-diol | wild-type, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
0.15 | - |
Propane-1,2-diol | mutant S224A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
0.16 | - |
Propane-1,2-diol | mutant T172S, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
0.51 | - |
Propane-1,2-diol | mutant S224C, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
1.9 | - |
Propane-1,2-diol | mutant S224N, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | Q59470 | alpha subunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propane-1,2-diol | - |
Klebsiella oxytoca | propanal + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
Propane-1,2-diol | mutant T172A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
12 | - |
Propane-1,2-diol | mutant S224C, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
17 | - |
Propane-1,2-diol | mutant S224N, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
34 | - |
Propane-1,2-diol | mutant T172S, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
64 | - |
Propane-1,2-diol | mutant S224A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
336 | - |
Propane-1,2-diol | wild-type, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | Km value 0.15 mM for wild-type | Klebsiella oxytoca |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
Propane-1,2-diol | mutant S224N, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
16 | - |
Propane-1,2-diol | mutant T172A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
24 | - |
Propane-1,2-diol | mutant S224C, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
210 | - |
Propane-1,2-diol | mutant T172S, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
430 | - |
Propane-1,2-diol | mutant S224A, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca | |
2200 | - |
Propane-1,2-diol | wild-type, pH not specified in the publication, temperature not specified in the publication | Klebsiella oxytoca |