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Literature summary for 4.2.1.28 extracted from

  • Tobimatsu, T.; Nishiki, T.; Morimoto, M.; Miyata, R.; Toraya, T.
    Low-solubility glycerol dehydratase, a chimeric enzyme of coenzyme B(12)-dependent glycerol and diol dehydratases (2008), Arch. Microbiol., 191, 199-206.
    View publication on PubMed
Search for more literature for 4.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
beta-subunit DNA and amino acid sequence determination and analysis, sequence comparison, and expression of wild-type and chimeric mutants, in Escherichia coli strain BL21(DE3), distribution of the chimeric mutants in recombinant bacterial cells, overview Klebsiella pneumoniae
beta-subunit DNA and amino acid sequence determination and analysis, sequence comparison, and expression of wild-type and chimeric mutants, in Escherichia coli strain BL21(DE3), distribution of the chimeric mutants in recombinant bacterial cells, overview Salmonella enterica
beta-subunit DNA and amino acid sequence determination and analysis, sequence comparison, and expression of wild-type and chimeric mutants, in Escherichia coli strain BL21(DE3), distribution of the chimeric mutants in recombinant bacterial cells, overview Klebsiella oxytoca

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric enzymes with subunit compositions alphaGbetaD2GgammaG and alphaGbetaGgammaDG, overview Klebsiella pneumoniae
additional information construction of chimeric enzymes with subunit compositions alphaGbetaD2GgammaG and alphaGbetaGgammaDG, overview Salmonella enterica
additional information construction of chimeric enzymes with subunit compositions alphaGbetaD2GgammaG and alphaGbetaGgammaDG, overview Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella pneumoniae the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes ?
-
 ?
additional information Salmonella enterica the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes ?
-
 ?
additional information Klebsiella oxytoca the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca
-
-
-
Klebsiella pneumoniae
-
-
-
Salmonella enterica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol
-
 Klebsiella pneumoniae propanal + H2O
-
 ?
1,2-propanediol
-
 Salmonella enterica propanal + H2O
-
 ?
1,2-propanediol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
additional information the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes Klebsiella pneumoniae ?
-
 ?
additional information the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes Salmonella enterica ?
-
 ?
additional information the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes Klebsiella oxytoca ?
-
 ?

Synonyms

Synonyms Comment Organism
diol dehydratase
-
 Klebsiella pneumoniae
diol dehydratase
-
 Salmonella enterica
diol dehydratase
-
 Klebsiella oxytoca
DL-1,2-propanediol hydro-lyase
-
 Klebsiella pneumoniae
DL-1,2-propanediol hydro-lyase
-
 Salmonella enterica
DL-1,2-propanediol hydro-lyase
-
 Klebsiella oxytoca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Klebsiella pneumoniae
37
-
 assay at Salmonella enterica
37
-
 assay at Klebsiella oxytoca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Klebsiella pneumoniae
8
-
 assay at Salmonella enterica
8
-
 assay at Klebsiella oxytoca