Literature summary for 4.2.1.28 extracted from

Sauvageot, N.; Pichereau, V.; Louarme, L.; Hartke, A.; Auffray, Y.; Laplace, J.M.
Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides (2002), Eur. J. Biochem., 269, 5731-5737.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli of the three genes encoding the subunits of the enzyme. Recombinant protein cannot form an active complex	Secundilactobacillus collinoides

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	10 mM, complete inhibition	Secundilactobacillus collinoides
cyanocobalamin	competitive inhibitor	Secundilactobacillus collinoides
glycerol	suicide inhibitor. The linear increase in beta-hydroxypropionaldehyde formation ceases after 4 min	Secundilactobacillus collinoides
Mg2+	10 mM, slight inhibitor	Secundilactobacillus collinoides
additional information	no inhibition with Li+ and NH4+	Secundilactobacillus collinoides
Na+	10 mM, complete inhibition	Secundilactobacillus collinoides

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008	-	coenzyme B12	-	Secundilactobacillus collinoides
1.6	-	1,2-propanediol	-	Secundilactobacillus collinoides
5.5	-	1,2-ethanediol	-	Secundilactobacillus collinoides
8.3	-	glycerol	-	Secundilactobacillus collinoides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22000	-	alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE	Secundilactobacillus collinoides
28000	-	alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE	Secundilactobacillus collinoides
63000	-	alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE	Secundilactobacillus collinoides
207000	-	gel filtration	Secundilactobacillus collinoides

Organism

Organism	UniProt	Comment	Textmining
Secundilactobacillus collinoides	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and gel filtration	Secundilactobacillus collinoides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.22	-	37ºC, pH 7.2, crude extract	Secundilactobacillus collinoides
0.77	-	37ºC, pH 7.2, purified enzyme	Secundilactobacillus collinoides

Storage Stability

Storage Stability	Organism
-20ºC, stable	Secundilactobacillus collinoides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-ethanediol	-	Secundilactobacillus collinoides	acetaldehyde	-	?
1,2-propanediol	-	Secundilactobacillus collinoides	propanal	-	?
glycerol	-	Secundilactobacillus collinoides	beta-hydroxypropionaldehyde	-	?

Subunits

Subunits	Comment	Organism
hexamer	alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE	Secundilactobacillus collinoides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.7	-	-	Secundilactobacillus collinoides

pH Range

pH Minimum	pH Maximum	Comment	Organism
8.5	9.2	-	Secundilactobacillus collinoides

Cofactor

Cofactor	Comment	Organism	Structure
coenzyme B12	adenosylcobalamin	Secundilactobacillus collinoides

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0264	-	cyanocobalamin	-	Secundilactobacillus collinoides

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Release 2023.1 (January 2023)