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Literature summary for 4.2.1.28 extracted from

  • Tobimatsu, T.; Sakai, T.; Hashida, Y.; Mizoguchi, N.; Miyoshi, S.; Toraya, T.
    Heterologous expression, purification, and properties of diol dehydratase, an adenosylcobalamin-dependent enzyme of Klebsiella oxytoca (1997), Arch. Biochem. Biophys., 247, 132-140.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 1,2-propanediol membrane-associated enzyme Klebsiella pneumoniae
0.56
-
 1,2-ethanediol membrane-associated enzyme Klebsiella pneumoniae
0.73
-
 glycerol membrane-associated enzyme Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane released from membrane at 1% Brij 35 Klebsiella pneumoniae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 alpha2beta2gamma2, 2 * 60000, 2 * 30000, 2 * 19000, SDS-PAGE, recombinant enzyme, beta2 is F component, alpha2gamma2 is S component, mutual effects on folding Klebsiella pneumoniae
30000
-
 alpha2beta2gamma2, 2 * 60000, 2 * 30000, 2 * 19000, SDS-PAGE, recombinant enzyme, beta2 is F component, alpha2gamma2 is S component, mutual effects on folding Klebsiella pneumoniae
60000
-
 alpha2beta2gamma2, 2 * 60000, 2 * 30000, 2 * 19000, SDS-PAGE, recombinant enzyme, beta2 is F component, alpha2gamma2 is S component, mutual effects on folding Klebsiella pneumoniae
220000
-
 gel filtration, membrane-associated recombinant enzyme Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
106
-
 membrane-associated enzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol
-
 Klebsiella pneumoniae propanal
-
 ?

Subunits

Subunits Comment Organism
hexamer alpha2beta2gamma2, 2 * 60000, 2 * 30000, 2 * 19000, SDS-PAGE, recombinant enzyme, beta2 is F component, alpha2gamma2 is S component, mutual effects on folding Klebsiella pneumoniae
More N-terminal amino acid analysis, role of cysteines Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
cobamide Km: 0.00083 mM, membrane-bound enzyme Klebsiella pneumoniae