Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | stimulates | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, crystals of the enzyme complex with levulinic acid solved at 1.67 A resolution, crystals belong to space group P42(1)2 with cell dimensions of a = b = 129.8 A, c = 86.7 A | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2-difluorosuccinic acid | competitive | Pseudomonas aeruginosa | |
diethyl dicarbonate | - |
Pseudomonas aeruginosa | |
EDTA | activity can be completely restored by addition of Mg2+ or Mn2+. Co2+, Zn2+, and Ni2+ partially restore EDTA-inhibited activity | Pseudomonas aeruginosa | |
levulinic acid | - |
Pseudomonas aeruginosa | |
protoporphyrin IX | - |
Pseudomonas aeruginosa | |
pyridoxal phosphate | - |
Pseudomonas aeruginosa | |
succinic acid | noncompetitive | Pseudomonas aeruginosa | |
succinic acid monomethyl ester | competitive | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
5-aminolevulinate | pH 8.6 in Na-Hepes buffer | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | partially restores activity after inhibition with EDTA | Pseudomonas aeruginosa | |
K+ | stimulates | Pseudomonas aeruginosa | |
Li+ | stimulates | Pseudomonas aeruginosa | |
Mg2+ | can completrly restore activity after inhibition by EDTA | Pseudomonas aeruginosa | |
Mg2+ | can completrly restore activity after inhibition by EDTA, stabilizes the oligomeric state but is not essential for octamer formation | Pseudomonas aeruginosa | |
Na+ | stimulates | Pseudomonas aeruginosa | |
Ni2+ | partially restores activity after inhibition with EDTA | Pseudomonas aeruginosa | |
Zn2+ | partially restores activity after inhibition with EDTA | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37832 | - |
6 * 37832, electrospray ionization mass spectrometry | Pseudomonas aeruginosa |
280000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminolevulinate + 5-aminolevulinate | - |
Pseudomonas aeruginosa | porphobilinogen + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 6 * 37832, electrospray ionization mass spectrometry | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
in presence of Mg2+ and K+ | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.8 | pH 7.5: about 35% of maximal activity, pH 9.8: about 50% of maximal activity | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
protoporphyrin IX | - |
Pseudomonas aeruginosa | |
0.03 | - |
diethyl dicarbonate | - |
Pseudomonas aeruginosa | |
0.03 | - |
pyridoxal phosphate | - |
Pseudomonas aeruginosa | |
1.5 | - |
levulinic acid | - |
Pseudomonas aeruginosa | |
1.9 | - |
succinic acid monomethyl ester | - |
Pseudomonas aeruginosa | |
7 | - |
2,2-difluorosuccinic acid | - |
Pseudomonas aeruginosa | |
9 | - |
succinic acid | - |
Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing, pH-range 3-10 | - |
5.4 |