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Literature summary for 4.2.1.24 extracted from

  • Frankenberg, N.; Heinz, D.W.; Jahn, D.
    Production, purification, and characterization of a Mg2+-responsive porphobilinogen synthase from pseudomonas aeruginosa (1999), Biochemistry, 38, 13968-13975.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NH4+ stimulates Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, crystals of the enzyme complex with levulinic acid solved at 1.67 A resolution, crystals belong to space group P42(1)2 with cell dimensions of a = b = 129.8 A, c = 86.7 A Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2,2-difluorosuccinic acid competitive Pseudomonas aeruginosa
diethyl dicarbonate
-
Pseudomonas aeruginosa
EDTA activity can be completely restored by addition of Mg2+ or Mn2+. Co2+, Zn2+, and Ni2+ partially restore EDTA-inhibited activity Pseudomonas aeruginosa
levulinic acid
-
Pseudomonas aeruginosa
protoporphyrin IX
-
Pseudomonas aeruginosa
pyridoxal phosphate
-
Pseudomonas aeruginosa
succinic acid noncompetitive Pseudomonas aeruginosa
succinic acid monomethyl ester competitive Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
5-aminolevulinate pH 8.6 in Na-Hepes buffer Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ partially restores activity after inhibition with EDTA Pseudomonas aeruginosa
K+ stimulates Pseudomonas aeruginosa
Li+ stimulates Pseudomonas aeruginosa
Mg2+ can completrly restore activity after inhibition by EDTA Pseudomonas aeruginosa
Mg2+ can completrly restore activity after inhibition by EDTA, stabilizes the oligomeric state but is not essential for octamer formation Pseudomonas aeruginosa
Na+ stimulates Pseudomonas aeruginosa
Ni2+ partially restores activity after inhibition with EDTA Pseudomonas aeruginosa
Zn2+ partially restores activity after inhibition with EDTA Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37832
-
6 * 37832, electrospray ionization mass spectrometry Pseudomonas aeruginosa
280000
-
gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminolevulinate + 5-aminolevulinate
-
Pseudomonas aeruginosa porphobilinogen + 2 H2O
-
?

Subunits

Subunits Comment Organism
octamer 6 * 37832, electrospray ionization mass spectrometry Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
in presence of Mg2+ and K+ Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.8 pH 7.5: about 35% of maximal activity, pH 9.8: about 50% of maximal activity Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0025
-
protoporphyrin IX
-
Pseudomonas aeruginosa
0.03
-
diethyl dicarbonate
-
Pseudomonas aeruginosa
0.03
-
pyridoxal phosphate
-
Pseudomonas aeruginosa
1.5
-
levulinic acid
-
Pseudomonas aeruginosa
1.9
-
succinic acid monomethyl ester
-
Pseudomonas aeruginosa
7
-
2,2-difluorosuccinic acid
-
Pseudomonas aeruginosa
9
-
succinic acid
-
Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa isoelectric focusing, pH-range 3-10
-
5.4