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Literature summary for 4.2.1.22 extracted from

  • Majtan, T.; Liu, L.; Carpenter, J.F.; Kraus, J.P.
    Rescue of cystathionine beta-synthase (CBS) mutants with chemical chaperones: purification and characterization of eight CBS mutant enzymes (2010), J. Biol. Chem., 285, 15866-15873.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine activity increases in the presence of Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
eight CBS mutants are expressed in Escherichia coli in the presence of chemical chaperones such as ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide Homo sapiens

Protein Variants

Protein Variants Comment Organism
A114V the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
E176K the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant does not respond to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
I435T the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
P422L the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
P49L the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
P78R the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
R125Q the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant does not respond to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
S466L the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + L-homocysteine
-
 Homo sapiens L-cystathionine + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer native gel Western blot Homo sapiens

Synonyms

Synonyms Comment Organism
CBS
-
 Homo sapiens
cystathionine beta-synthase
-
 Homo sapiens