Cloned (Comment) | Organism |
---|---|
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli | Pyrococcus furiosus |
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli | Thermoplasma acidophilum |
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli | Pyrococcus horikoshii |
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli | Thermoplasma volcanium |
Crystallization (Comment) | Organism |
---|---|
crystal structures of both mercury- and iron-bound TA0289 (1.52.0 A resolution) reveals a dimeric protein whose intersubunit contacts are formed exclusively by the alpha-helices of two cystathionine beta-synthase subdomains, whereas the C-terminal domain has a classical Zn ribbon planar architecture | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains 0.38 Ca atoms/subunit | Pyrococcus furiosus | |
Fe | contains 0.13 Fe atoms/subunit | Thermoplasma volcanium | |
Fe | contains 0.67 Fe atoms/subunit, in minimal medium | Thermoplasma acidophilum | |
Fe2+ | contains 0.16 Fe atoms/subunit | Pyrococcus furiosus | |
Zn | contains 0.17 Zn atoms/subunit, in minimal medium | Thermoplasma acidophilum | |
Zn | contains 0.25 Zn atoms/subunit | Thermoplasma volcanium | |
Zn2+ | contains 0.35 Zn atoms/subunit | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19700 | - |
2 * 19700, calculated from sequence | Thermoplasma volcanium |
20200 | - |
3 * 20200, calculated from sequence | Pyrococcus furiosus |
20300 | - |
2 * 20300, calculated from sequence | Thermoplasma acidophilum |
36800 | - |
gel filtration | Thermoplasma volcanium |
38000 | - |
gel filtration | Thermoplasma acidophilum |
62800 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Pyrococcus horikoshii | - |
- |
- |
Thermoplasma acidophilum | - |
- |
- |
Thermoplasma volcanium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli | Pyrococcus furiosus |
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli | Thermoplasma acidophilum |
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli | Pyrococcus horikoshii |
N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli | Thermoplasma volcanium |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 19700, calculated from sequence | Thermoplasma volcanium |
dimer | 2 * 20300, calculated from sequence | Thermoplasma acidophilum |
trimer | 3 * 20200, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PF1953 | - |
Pyrococcus furiosus |
PH0267 | - |
Pyrococcus horikoshii |
TA0289 | - |
Thermoplasma acidophilum |
TV1335 | - |
Thermoplasma volcanium |