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Literature summary for 4.2.1.22 extracted from

  • Frank, N.; Kent, J.O.; Meier, M.; Kraus, J.P.
    Purification and characterization of the wild type and truncated human cystathionine beta-synthase enzymes expressed in E. coli (2007), Arch. Biochem. Biophys., 470, 64-72.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine stimulates Homo sapiens
S-adenosylhomocysteine stimulates activity 1.1fold at 0.48 mM Homo sapiens
sinefungin stimulates activity 1.28fold at 1 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild type and truncated human cystathionine beta-synthase enzyme in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 L-homocysteine 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine Homo sapiens
1.04
-
 L-homocysteine 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine Homo sapiens
1.41
-
 L-serine 37°C, pH 8.0, wild-type enzyme, without -adenosyl-L-methionine Homo sapiens
2.13
-
 L-serine 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type and truncated human cystathionine beta-synthase expressed in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
 wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + L-homocysteine
-
 Homo sapiens cystathionine + H2O
-
 ?
O-acetyl-L-serine + L-homocysteine poor substrate for both the wild type and the N- and C-terminally truncated enzyme 71–400 CBS Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 L-serine 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine Homo sapiens
3.3
-
 L-homocysteine 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine Homo sapiens
3.67
-
 L-serine 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine Homo sapiens
4.66
-
 L-homocysteine 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine Homo sapiens
12.7
-
 L-homocysteine 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine Homo sapiens
14.01
-
 L-serine 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 broad, full-length and the C-terminally truncated enzyme (CBS 1-413) Homo sapiens
9
-
 sharp, N- and C-terminally truncated enzyme (BS 71-400) Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.5 pH 7.3: about 45% of maximal activity, pH 9.5: about 80% of maximal activity, full-length and the C-terminally truncated enzyme (CBS 1-413) Homo sapiens
8.3 9.5 pH 8.3: about 45% of maximal activity, pH 9.5: about 85% of maximal activity N- and C-terminally truncated enzyme (BS 71-400) Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme cystathionine beta-synthase binds one mole of heme per subunit Homo sapiens