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Literature summary for 4.2.1.22 extracted from

  • Janosik, M.; Kery, V.; Gaustadnes, M.; Maclean, K.N.; Kraus, J.P.
    Regulation of human cystathionine beta-synthase by S-adenosyl-L-methionine: evidence for two catalytically active conformations involving an autoinhibitory domain in the C-terminal region (2001), Biochemistry, 40, 10625-10633.
    View publication on PubMed
Search for more literature for 4.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine induces conformational change Homo sapiens

Application

Application Comment Organism
medicine inherited deficiency leads to homocystinuria, an autosomal recessivlely inherited disease of sulfur metabolism Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
I435T inducible by S-adenosyl-L-methionine, but less responsive than wild-type enzyme to physiologically relevant concentrations Homo sapiens
S466L not inducible by S-adenosyl-L-methionine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 homocysteine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
0.08
-
 homocysteine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
0.1
-
 homocysteine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
0.17
-
 homocysteine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
0.2
-
 homocysteine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
3.6
-
 L-serine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2
-
 L-serine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.5
-
 L-serine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.9
-
 L-serine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
7.2
-
 L-serine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
486000
-
 octamer, gel filtration Homo sapiens
1330000
-
 multimer, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two patient-derived forms S466L and I435T, 95% purity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Serine + homocysteine
-
 Homo sapiens Cystathionine + H2O
-
 r

Subunits

Subunits Comment Organism
multimer wild-type enzyme and I435T mutant, gel filtration Homo sapiens
octamer wild-type enzyme and I435T mutant, gel filtration Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 melting point of wild type and S466L mutant Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
 L-serine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
5.9
-
 L-serine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
5.9
-
 homocysteine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
6.2
-
 homocysteine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
14.6
-
 L-serine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
15.5
-
 homocysteine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
15.8
-
 L-serine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
19.7
-
 L-serine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
32.1
-
 homocysteine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
34
-
 homocysteine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens