KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
homocysteine | enzyme composed of MW 48000 subunits | Rattus norvegicus | |
4 | - |
L-Ser | - |
Rattus norvegicus | |
25 | - |
homocysteine | enzyme composed of MW 68000 subunits | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, proteolytically activated enzyme, derived from 4 * 68000 enzyme, SDS-PAGE | Rattus norvegicus |
68000 | - |
2 * 48000, proteolytically activated enzyme, derived from 4 * 68000 enzyme, SDS-PAGE | Rattus norvegicus |
68000 | - |
4 * 68000, enzyme from fresh liver extract, SDS-PAGE | Rattus norvegicus |
100000 | - |
aged enzyme extract, gel filtration | Rattus norvegicus |
250000 | - |
fresh enzyme extract, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Serine + homocysteine | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the tetrameric enzyme, MW 4 * 68000, is processed to a dimeric (2 * 48000) enzyme by proteolysis, 60fold increase of activity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Serine + homocysteine | - |
Rattus norvegicus | Cystathionine + H2O | - |
? | |
L-Serine + homocysteine | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, proteolytically activated enzyme, derived from 4 * 68000 enzyme, SDS-PAGE | Rattus norvegicus |
tetramer | 4 * 68000, enzyme from fresh liver extract, SDS-PAGE | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement | Rattus norvegicus |