Crystallization (Comment) | Organism |
---|---|
in complex with inhibitor N-(4'-trifluoro-methoxybenzenesulfonyl)-2-amino-ethyl phosphate | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(4'-trifluoro-methoxybenzenesulfonyl)-2-amino-ethyl phosphate | tight binding analogue of 1-C-(indol-3-yl)glycerol 3-phosphate which binds to the alpha-subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | P00929 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | P00929 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
TrpA | alpha-chain | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | residue Lys87 of the beta-subunit activates C4' of pyridoxal 5'-phosphate for substrate nucleophilic attack | Salmonella enterica subsp. enterica serovar Typhimurium |
General Information | Comment | Organism |
---|---|---|
physiological function | residue Lys87 of the beta subunit plays multiple catalytic roles, it bonds to the pyridoxal phosphate cofactor, activates C4' for nucleophilic attack via a protonated Schiff base nitrogen, and abstracts and returns protons to pyridoxal phosphate-bound substrates. The protonation state of the epsilon-amino group switches between protonated and neutral states as the beta-subunit undergoes conversion from one intermediate to the next during catalysis | Salmonella enterica subsp. enterica serovar Typhimurium |