Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Shewanella frigidimarina |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1M HEPES-Na, pH 7.5, 2% (v/v) polyethylene glycol 400 and 2 M ammonium sulfate, at 4°C | Shewanella frigidimarina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate | Shewanella frigidimarina | - |
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate | Shewanella frigidimarina K14-2 | - |
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella frigidimarina | - |
- |
- |
Shewanella frigidimarina K14-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
SuperQ-650M column chromatography, Sephacryl S-300HR column chromatography, and MonoQ HR5/5 column chromatography | Shewanella frigidimarina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-C-(indol-3-yl)glycerol 3-phosphate | - |
Shewanella frigidimarina | D-glyceraldehyde 3-phosphate + indole | - |
? | |
1-C-(indol-3-yl)glycerol 3-phosphate | - |
Shewanella frigidimarina K14-2 | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate | - |
Shewanella frigidimarina | L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate | - |
Shewanella frigidimarina K14-2 | L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tryptophan synthase alpha subunit | - |
Shewanella frigidimarina |
TSA | - |
Shewanella frigidimarina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42.5 | 45 | - |
Shewanella frigidimarina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
the denaturation temperature of the enzyme is 56°C at pH 7.0 | Shewanella frigidimarina |