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Literature summary for 4.2.1.20 extracted from
- Novel allosteric effectors of the tryptophan synthase alpha(2)beta(2) complex identified by computer-assisted molecular modeling (2000), Biochim. Biophys. Acta, 1476, 287-299.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Indole-3-acetamide | allosteric effector, binds to the alpha-subunit, slightly activating effect on the enzyme complex, stabilization of the alpha-aminoacrylate Schiff base by perturbing the equilibrium of the catalytic intermediates formed at the beta-active site | Salmonella enterica subsp. enterica serovar Typhimurium |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycerol 3-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Indole-3-acetamide | allosteric effector, binds to the alpha-subunit, inhibitory on the alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
| indole-3-acetyl-L-alanine | allosteric effector, binds to the beta-subunit | Salmonella enterica subsp. enterica serovar Typhimurium | |
| indole-3-acetyl-L-aspartate | allosteric effector, binds to the alpha-subunit, slightly inhibitory effect on the enzyme complex, stabilization of the alpha-aminoacrylate Schiff base by perturbing the equilibrium of the catalytic intermediates formed at the beta-active site | Salmonella enterica subsp. enterica serovar Typhimurium | |
| indole-3-acetyl-L-valine | allosteric effector, binds to the beta-subunit | Salmonella enterica subsp. enterica serovar Typhimurium | |
| indole-3-acetylglycine | allosteric effector, binds to the alpha-subunit, slightly inhibitory effect on the enzyme complex, stabilization of the alpha-aminoacrylate Schiff base by perturbing the equilibrium of the catalytic intermediates formed at the beta-active site | Salmonella enterica subsp. enterica serovar Typhimurium | |
| indole-3-acrylate | allosteric effector, binds to the beta-subunit, inhibitory on the alpha-reaction due to high concentrations of indole and glyceraldehyde 3-phosphate in the assay mixture | Salmonella enterica subsp. enterica serovar Typhimurium | |
| indole-3-lactate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), mechanisms of alpha-and beta-reaction, active site structures | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
| L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
| additional information | functional properties of one subunit are allosterically regulated by ligands of the other subunit, computer-assisted molecular modeling, alpha-subunit ligands do not bind to beta-subunit | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2beta2 enzyme complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
indole-3-acrylate | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.45 | - |
Indole-3-acetamide | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.48 | - |
indole-3-acetyl-L-valine | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.48 | - |
indole-3-lactate | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.95 | - |
indole-3-acetyl-L-alanine | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.2 | - |
indole-3-acetylglycine | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.67 | - |
indole-3-acetyl-L-aspartate | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 12.8 | - |
glycerol 3-phosphate | pH 7.8, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
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